Fig. 3: EBB3 is primarily expressed in the shoot apex and differentially regulated during the dormancy cycle and bud-break.
From: EARLY BUD-BREAK 1 and EARLY BUD-BREAK 3 control resumption of poplar growth after winter dormancy
a Relative expression of EBB3 in different tissues of WT-717 plants. b Relative expression of EBB3 in vegetative buds of wild-growing aspen (Populus tremuloides) trees. c Relative expression of EBB3 in WT-T89 plants under manipulative dormancy treatments in controlled growth chamber conditions. Expression values are the average of three biological replicates ±SEM, normalized to the reference genes ACT7 for WT-717 clones, and UBQ for WT-T89 clones, respectively. 0 W: time point before inductive treatments, 10 WSD: 10 weeks under SD photoperiod, 2WC: 2 weeks under cold, 5WC: 5 weeks under cold, 2WLD: 2 weeks under LD photoperiod and warm temperature. d Average H3K27me3 abundance of three biological replicates in the EBB3 locus, including 2 Kb downstream and upstream regions, before dormancy inductive treatments (0 W) and during dormancy induction, (6WSD, 10WSD) and dormancy release (4WC). H3K27me3 abundance was normalized relative to H3 abundance. Shaded areas indicate ±standard deviation. TSS transcription start site, TTS transcription termination site, cds coding sequence, UTR untranslated region. Source data underlying Fig. 3c are provided as a Source Data file.
