Fig. 2: Conditional Nedd4l deficiency inhibited antiviral innate immunity in vivo. | Nature Communications

Fig. 2: Conditional Nedd4l deficiency inhibited antiviral innate immunity in vivo.

From: E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3

Fig. 2: Conditional Nedd4l deficiency inhibited antiviral innate immunity in vivo.

a ELISA assay of IFN-β, IL-6, and TNF-α in serum of control Nedd4lfp/fpLyz2-Cre−/− and Nedd4lfp/fpLyz2-Cre+/+ mice intraperitoneally infected with VSV (4 × 105 PFU/g) for 6 and 12 h (n = 8 per group). b, c RT-qPCR analysis of VSV mRNA and TCID50 in spleen (b) and liver (c) in control Nedd4lfp/fpLyz2-Cre−/− and Nedd4lfp/fpLyz2-Cre+/+ mice intraperitoneally infected with VSV (4 × 105 PFU/g) for 24 h (RT-qPCR analysis n = 8 and TCID50 n = 6). d The survival rates of control Nedd4lfp/fpLyz2-Cre−/− and Nedd4lfp/fpLyz2-Cre+/+ mice were monitored for 3 days after intraperitoneally infected with VSV (8 × 105 PFU/g) (Nedd4lfp/fpLyz2-Cre−/− n = 7 and Nedd4lfp/fpLyz2-Cre+/+ n = 8, and p-values by two-tailed unpaired Student’s t-test are indicated). Data are presented as mean ± SD and p-values by two-tailed unpaired Student’s t-test are indicated in (a–c) and p-value by Gehan-Breslow-Wilcoxon test are indicated in (d). Results in (a–d) are representative of three independent experiments.

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