Fig. 4: Mouse mitochondrial ND5 G12918A mutation induced by DdCBE.
From: Mitochondrial DNA editing in mice with DddA-TALE fusion deaminases
a The DdCBE target for generating the m.G12918A point mutation, which would create a D393N change in the ND5 protein. The target codon is underlined and the possible editing locus is shown in red. b The efficiency of cytosine-to-thymine base editing with DdCBE in NIH3T3 cells. The annotations indicate the combination of DdCBE pairs that were co-transfected. Error bars are s.e.m. for n = 3 biologically independent samples (n.s. not significant, *p < 0.05, and **p < 0.01 using Student’s two-tailed t test). P values of left-G1333-N + right-G1333-C, left-G1333-C + right-G1333-N, left-G1397-N + right-G1397-C, and left-G1397-C + right-G1397-N for C6 mutation are 0.0052, 0.0099, 0.0027, and 0.0040, respectively. P values for n.s. is 0.4971. c m.G12918A point mutation base editing efficiency in mouse blastocysts. The sequencing data were obtained from cultured blastocysts that developed after one-cell stage embryos were microinjected with mRNA encoding the left-G1397-C and right-G1397-N DdCBE. d Mice (F0) carrying an ND5 point mutation. F0 pups, which harbor an ND5 point mutation, that developed after microinjection of the DdCBE mRNAs. Corresponding alignment of mutant sequences from newborn pups. Edited bases are shown in red, and the column on the right indicates the editing frequencies in the mutant mitochondrial genome. Source data are provided in the Source data file.
