Fig. 5: Newly identified FGM proteins localize to nascent centrioles and cilia in diverse centriole amplification stages.
From: Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly
a–g Representative 3D-SIM images for the indicated FGM proteins (green) in mTECs fixed at day 3. White arrows indicate parental centrioles, one of which was magnified. White arrowheads and frames respectively indicate typical regions containing deuterosomes and basal bodies that were magnified to show details. Yellow arrowheads in the color-coded insets point to immunofluorescent signals related to typical nascent centrioles. The diagrams illustrate FGM protein localizations in stage-I parental centrioles and during the deuterosome-mediated centriole biogenesis (II–VI). h, i Cep131 localized to a novel Centrin-positive structure protruded from the center of transition zone into cilia. Shown are typical basal bodies in stage-VI mEPCs at day 3. Cep152 labeled the centriolar proximal side. Cep290 served as transition zone marker. The constrictions of Centrin streaks are marked by dashed lines. Note that Cep131 localized to the region of Centrin streaks above the constriction and sometimes also displayed additional punctate immunofluorescent signals around the basal bodies (h).
