Fig. 1: The localization of mammalian VPS13D. | Nature Communications

Fig. 1: The localization of mammalian VPS13D.

From: An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D−TSG101 interactions

Fig. 1

a qPCR assays confirmed the efficiency and specificity of siRNAs-mediated VPS13D suppression in HEK293 cells. Two-tailed unpaired student’s t-test. Mean ± SD. b Western blots demonstrated the efficiency of siRNAs-mediated VPS13D suppression in HEK293 cells transfected with VPS13D^sfGFP with anti-GFP and anti-tubulin antibody. c Confocal image of fixed HEK293 cells stained with pre-cleared anti-VPS13D antibody (red), BODIPY493/503 (LD; green), and anti-CoxIV antibody (mitochondria; blue) upon treatments with either scrambled (top panel) or VPS13D siRNAs (bottom panel). Left: merged image of whole cells; right: three insets from boxed regions in whole cell image. Contrast range of images was set to the same level for both scrambled and VPS13D siRNAs treated cells. Yellow arrows denoted VPS13D puncta at potential mitochondria-LD junctions. Four independent imaging sessions were performed with similar results. d Percentage of VPS13D puncta (n = 644 from 20 cells in four independent assays) present at mitochondria-LD junctions upon OA/EBSS treatment. Mean ± SD. e Percentage of mitochondria-LD junctions (n = 286 from 20 cells in four independent assays) marked with VPS13D puncta upon OA/EBSS treatment. Mean ± SD. Scale bar, 10 μm in whole cell image and 2 μm in insets in c.

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