Fig. 3: The VPS13D N-terminal region targets mitochondria. | Nature Communications

Fig. 3: The VPS13D N-terminal region targets mitochondria.

From: An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D−TSG101 interactions

Fig. 3

a Diagram of truncation mutants of the VPS13D N-terminal region. b Confocal image of a COS7 cell expressing VPS13D (404-913)-GFP in CM without OA (top panel) or with OA (bottom panel). c Confocal image of a COS7 cell stably expressing VPS13D_N-GFP (green), mitoBFP (blue) and ACSL3-Halo (magenta) in CM without OA (top panel) or with OA (bottom panel). One inset from a boxed region was shown at bottom left. d Western blots demonstrated the distribution of the VPS13D_N-GFP protein in subcellular fractions in COS7 cells as in c S2/ P2: 7000 g supernatant and pellet; S3/P3: 20,000 g supernatant and pellet. e Confocal image of a HEK293 cell expressing VPS13D_N-GFP in CM without OA (top panel) or with OA (bottom panel). One inset from a boxed region was shown at bottom left. f Confocal image of a COS7 cell expressing GFP-VPS13D-ΔN in CM without OA. Yellow arrows indicated LDs with GFP-VPS13D-ΔN while white arrows denoting mitochondria not associating with GFP-VPS13D-ΔN. g Confocal image of a COS7 cell expressing GFP-VPS13D-ΔN (L3991Q L4052Q L4053Q) in CM without OA. White arrows indicated the LDs not associating with GFP-VPS13D-ΔN (L3991Q L4052Q L4053Q). Scale bar, 10 μm in whole cell image and 2 μm in insets in b, c, e, f and g.

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