Fig. 4: Smn interacts with 7SK/hnRNP complexes in the nucleus and cytosol.
From: Interaction of 7SK with the Smn complex modulates snRNP production
a Western blot analysis of NSC-34 subcellular fractions. Cyt, cytosol; Nuc, nuclear soluble proteins and organelles; Chr, chromatin-associated proteins; T, total lysate. b Quantification of Western blot signals in a. Data are mean ± s.d. (n = 3). c Quantification of 7SK RNA levels by qPCR. Data are mean ± s.d. (n = 4). d Western blot analysis of proteins co-immunoprecipitated by anti-Larp7 from subcellular fractions of NSC-34 cells. Fractions were pretreated with RNase as indicated. e Same as in d but with anti-Smn. Source data are provided as a Source Data file.
