Fig. 5: Depletion of individual components of the Smn complex alters its binding to Larp7.
From: Interaction of 7SK with the Smn complex modulates snRNP production
a Western blot analysis of proteins co-immunoprecipitated by anti-Larp7 from control and SmB/B’ knockdown (shSnrpb) NSC-34 cells. b Quantification of Western blot signals in a. Data are mean ± s.d.; *P ≤ 0.05; ***P ≤ 0.001; ****P ≤ 0.0001; unpaired two-tailed t-test (n = 3). c Co-immunoprecipitation of proteins by anti-Larp7 from control or Gemin2 knockdown (shGemin2) NSC-34 cells. d,e Co-immunoprecipitation of proteins by anti-Larp7 from control or Smn knockdown (shSmn) NSC-34 cells. f Co-immunoprecipitation of proteins by anti-SmB/B’ from brains of Smn+/+;SMN2 or Smn−/−;SMN2 mice. Note that the immunosignal at 25 kDa for mouse-IgG immunoprecipitation is non-specific from the antibody light chain. g Model of Smn interactions with 7SK/hnRNP R. Source data are provided as a Source Data file.
