Table 1 Key resources table.

Mouse HIF-1α

BD

610959

Rabbit PKM2

CellSignaling Technology

4053 S

Rabbit PHD3

Abcam

ab30782

Mouse hnRNPK

Santa cruz

Sc53620

Rabbit hnRNPF

Abcam

Ab50982

Mouse JMJD5

Abcam

Ab10639

Rabbit Hydroxyproline

Abcam

Ab37067

Rabbit LaminB1

Cell Signaling Technology

12255 S

Mouse MnSOD

BD

611580

Rabbit HIF-2α

Cell Signaling Technology

59973 S

Rabbit H3K27me3

Cell Signaling Technology

9733 S

Flag

Abcam

Ab205606

Mouse β-actin

abcam

Ab6276

Rabbit PKM1

proteintech

15821-1-AP

Biotin-antibody

Invitrogen

033700

Alexa Fluor Goat anti-mouse 594

Invitrogen

A11032

Alexa Fluor Goat anti-mouse 555

Invitrogen

A28180

Digoxin-antibody

Abcam

Ab419

Rabbit LDHA

Cell Signaling Technology

3582

Rabbit Ki67

Abcam

Ab15580

Rabbit GLUT1

Abcam

Ab115730

One Shot Stbl3

Invitrogen

C7373–03

pLentiCRISPR v2 plasmid

⁵⁷

18011CZ

pGL3-luciferase reporter vector

Jeneray biotech

VQP0124

PcDNA3.1 vector

Jeneray biotech

Addgene#69352

DH5α competent cells

TAKARA

9057

Puromycin

Gibco

A11138–03

10X T4 Ligation Buffer

New England Biolabs

#B0202

Pierce™ IP Lysis Buffer

Thermo Fisher Scientific

87787

Trizol

Invitrogen

15596–026

Dynabeads M-280 Streptavidin magnetic beads

Invitrogen

11205D

recombinant myc-labeled PHD3

Origene

TP310319

His-labeled PKM2

Origene

TP721212

EZ-Magna ChIP A/G Recombinant protein

Merck Millipore

17–10086

NEBNext Ultra II Q5 Master Mix

New England Biolabs

M0544L

Colorimetric peptide assay

Invitrogen

23275

16%Formaldehyde (w/v)

Thermo Fisher Scientific

28908

Pierce chip-grade protein A/G magenetics beads

Invitrogen

26162

Deoxynucleotide (dNTP) Solution Mix

New England Biolabs

N0447L

10 MM Bio-16-UTP 1 TUBE EACH

Invitrogen

AM8452

Recombinant RNasin® Ribonuclease Inhibitor

Promega

N2515

Halt™ Protease Inhibitor Cocktail (100X)

Pierce

87786

Superscript First-Strand cDNA Synthesis Kit

Invitrogen

18080–051

SYBR Premix Ex Taq II kit

TAKARA

DRR081A

DIG Luminescent Detection Kit for Nucleic Acids

Roche

11363514910

Cell fractionation assay PARIS^TM kit

Ambion

AM 1921

SMARTer RACE 5’/3’ Kit

Clontech

634860

Magna RNA Immunoprecipitation Kit

Millipore

17–700

TranscriptAid T7 High Yield Transcription Kit

Ambion

AM1333

MagnaChIP HiSens Chromatin IP Kit

Merck Millipore

17–10461

Lightshift^TMChemiluminescent RNA EMSA kit

Thermo Fisher Scientific

20158

Glucose Uptake Colorimetric Assay Kit

Biovision

k676

lactate Colorimetric/Fluorometric Assay kit

Biovision

k607

XFglycolytic stress test kit

Agilent Technologies

103020100

Human: MDA-MB-231

N/A

N/A

Human:MCF-7

N/A

N/A

Human:76 N

N/A

N/A

Human:293 T

N/A

N/A

Human: T47D

N/A

N/A

Human:SK-BR3

N/A

N/A

Human:BT549

N/A

N/A

Human:MDA-MB-468

N/A

N/A

Balb/c nu/nu mouse (3–4 weeks old female)

Vitonlihua Laboratory Animal Center

11400700211483

GampeRHIFAL LNA1

Exiqon

CATTCTGGGACGGAGA

GampeRHIFAL LNA2

Exiqon

GTAACATGGTGATAAT

GampeR HIF1α-1 LNA

Exiqon

TGGCAAGCATCCTGTA

GampeR Control LNA

Exiqon

AACACGTCTATACGC

LNA mRNA Detection Probe

Exiqon

ACGGAGTAATGTTGGGTAAGCT

beta-actin, hsa/mmu/rno, 5-dig labeled

Exiqon

E61201

HIFAL and hnRNPF sgRNA (See Table S1for sequences)

Invitrogen

N/A

Sequences of siRNAs (See Table S1 for sequences)

Invitrogen

N/A

Primers for qRT-PCR (See Table S1 for sequences)

Invitrogen

N/A

Primers for ChIP assay (See Table S1 for sequences)

Invitrogen

N/A

Primers for 5’ and 3’RACE (See Table S1 for sequences)

Invitrogen

N/A

ChIP-seq reads

http://www.bioinformatics.babraham.ac.uk/projects/fastqc/

⁵⁵

adapter sequences

http://hannonlab.cshl.edu/fastx_toolkit/index.html

⁵⁶

SPSS version 16.0

Chicago, IL

N/A

LaserConfocal Scan soft Zen2012

Carl Zeiss

N/A

TCGAbiolinks

http://bioconductor.org/packages/release/bioc/html/TCGAbiolinks.html

⁶¹

All spectra of modified peptides

http://prospector.ucsf.edu/prospector/cgi-bin/msform.cgi?form=msproduct

⁶²