Fig. 1: Waves of genome reorganization bookend plasmablast differentiation.

a Stages of B-cell activation and differentiation. b Multidimensional-scaling plot of RNA-seq data of B-cell activation. c Numbers of differentially expressed genes between B-cell activation stages calculated with fold changes significantly above 1.5 (Treat FDR < 0.05). Blue indicates a decrease in expression level with activation. Red indicates an increase in expression level with activation. d Multidimensional-scaling plot of B-cell activation demonstrating two dominant waves of genome organizational change. e Numbers of differential interactions (DIs) between B-cell activation stages. Blue indicates the weakening of organization with activation. Red indicates strengthening of organization with activation. f Normalized in situ HiC contact matrices at 50 kbp showing genome organization at the Twistnb (chr12:33.2–35.3 Mb) and Bcl6 (chr16:23.85–25.15 Mb) loci at each stage of B-cell activation. Color scale indicates the number of reads per bin pair. g Gene ontological terms with genes increasing in differential expression between the naive and 3 h activated B cells over-represented. Groups ranked according to the percentage of groups genes that are differentially expressed. h Gene ontological terms with genes increasing in differential expression between the 3 and 10 h activated B cells over-represented. Groups ranked according to the percentage of genes that are differentially expressed. i Gene ontological terms with genes increasing in differential expression between the 10 h activated B cells and those imminently to divide over-represented. Groups ranked according to the percentage of groups genes that are differentially expressed. Source data are provided as a Source Data file.