Fig. 1: Ythdc1 and Ythdf are the major m6A-binding proteins in Drosophila.
From: A neural m6A/Ythdf pathway is required for learning and memory in Drosophila
A RNA photoaffinity probes used in crosslinking assays. B and C Both nuclear (Ythdc1) and cytoplasmic (Ythdf) Drosophila YTH factors specifically recognize m6A within the AAm6ACU context. Point mutations of aromatic residues that line the m6A cage (3A variants) abolish selective binding to m6A probes. FT Flow through. Shown is one representative result from two repeats. D Proteomic profiling of S2 cells using m6A and A RNA photoaffiinity probes reveals Ythdc1 and Ythdf as the only preferentially bound (reader) proteins, and no strongly repelled proteins were found in these conditions. Background proteins are clustered together around the plot origin; threshold = 2X the interquartile range. Two biological replicates were plotted. E Selected values of fly homologs of mammalian m6A readers and repelled proteins expressed in S2 cells, as well as candidates of novel bound/repelled factors. Source data are provided as a Source Data file and Supplementary Dataset 1.
