Fig. 2: m6A pathway is required for short-term learning and memory (STM) in Drosophila.
From: A neural m6A/Ythdf pathway is required for learning and memory in Drosophila
A Schematic of the multifly olfactory training (MOT) chamber apparatus used for behavioral measurements. B Paradigm for shock-associated odor avoidance assay for STM acquisition. C and D Hemizygote null conditions for Mettl3[null] and Mettl3[Δcat] both led to STM impairment. Mettl3[null]/Df (n = 216) vs. Mettl3[null]/+ (n = 132) = −0.23[95CI −0.4, −0.06] p = 0.033. Mettl3[Δcat]/Df (n = 168) vs. Mettl3[Δcat]/+ (n = 144) = −0.35[95CI −0.6, −0.14] p = 0.017. E and F Hemizygote null conditions for Mettl14[fs] and Mettl14[sk1] led to a mild STM impairment. Mettl14[sk1]/Df (n = 120) vs. Mettl14[sk1]/+ (n = 120) = −0.19[95CI −0.52, +0.14] p = 0.345. Mettl14[fs]/Df (n = 168) vs. Mettl14[fs]/+ (n = 168) = −0.15[95CI −0.37, +0.06] p = 0.505. G Pan-neuronal expression of UAS-Mettl3 using elav-Gal4 (elav-G4) in Mettl3[null] hemizygotes rescued their STM phenotype. Mettl3[null]/+ (n = 192) vs. elav-G4/w1118 (n = 168) = +0.02[95CI −0.17, +0.21] p = 0.647. Mettl3[null]/Mettl3[Δcat] (n = 180) vs. elav-G4/w[1118] (n = 168) = −0.33[95CI −0.57, −0.07] p = 0.085. Mettl3[null]/Mettl3[Δcat] + elav-G4 UAS-Mettl3 (n = 180) vs. elav-G4/w[1118] (n = 168) = +0.08[95CI −0.1, +0.22] p = 0.132. All assays in C–F were conducted in 20-day-old flies. Bars present mean values. Each dot in the scatter plots represents a PI average of 12 flies. All control–test differences are displayed as effect sizes with error curves and 95% confidence intervals. No null-hypothesis significance testing was performed; two-tailed Mann–Whitney P values are shown for legacy purposes only. For multiple comparisons, several test groups were compared against a common control group. PI = performance index. Source data are provided as a Source Data file.
