Fig. 5: Validation of Drosophila m6A targets and their association with readers.
From: A neural m6A/Ythdf pathway is required for learning and memory in Drosophila
A–A′ m6A-RIP-qPCR validation of m6A transcripts in w[1118] control and Mettl3[null] knockout whole female flies. Error bars, mean ± SD; n = 3 biological replicates. Two-tailed t-test, *p < 0.05, **p < 0.01. syx1A p = 0.012, aqz p = 0.005, fl(2)d p = 0.004, pum p = 0.009, prosap p = 0.016, futsch p = 0.009, gish p = 0.044, sky p = 0.010. n.s. non-significant, fwe p = 0.604, CG7970 p = 0.085. B and C RIP-qPCR of m6A targets in S2-S cells and transfected Ythdc1 (B) or Ythdf (C) constructs shows specific pulldown of several targets relative to GFP-RIP control, and the association of several of these is compromised by mutation of the YTH domain (3A versions). Shown is one representative result from three independent repeats. Source data are provided as a Source Data file.
