Fig. 2: Characterizations of Rdots.
From: Ultra-bright Raman dots for multiplexed optical imaging
a Spontaneous Raman spectra of six Rdots generated using Carbow dyes. arb. units: arbitrary units. b Raman spectra of Rdots and free Carbow dyes in DMSO, showing no significant peak shift or broadening. arb. units: arbitrary units. c RIE and Raman shift of Rdots made by various alkyne and nitrile containing probes. Rdots were made with a large variety of compounds that contain alkyne or nitrile molecules, and their RIE (relative Raman intensity versus EdU) values and Raman shifts were measured. RIE values were measured with Spontaneous Raman spectrometer with 532 nm excitation wavelength, except MARS dye, which were evaluated with SRS microscopy. Probes indicated with colored stars were used for immunostaining in this study. PDDA: poly(deca-4,6-diynedioic acid)20, EdU: 5-ethynyl-2’-deoxyuridine. d Hydrodynamic diameter of PS nanoparticles before and after doping measured by DLS. The size distributions show no change when PS nanoparticles are doped with Raman dyes, and the resulting 21 nm Rdots are monodispersive. e Hydrodynamic diameter of Rdots after biofunctionalization.
