Fig. 3: EIF6 and TP53 mutations attenuate p53 activation via different mechanisms. | Nature Communications

Fig. 3: EIF6 and TP53 mutations attenuate p53 activation via different mechanisms.

From: Distinct genetic pathways define pre-malignant versus compensatory clonal hematopoiesis in Shwachman-Diamond syndrome

Fig. 3: EIF6 and TP53 mutations attenuate p53 activation via different mechanisms.

a Quantification of 80S:60S ratio from polysome profiles in SDS patient-derived primary fibroblasts transduced with shRNAs targeting luciferase, EIF6 (left panel) or TP53 (right panel). b OP-Puro incorporation in primary SDS patient-derived fibroblasts transduced with shRNAs targeting luciferase, EIF6 (left panel) or TP53 (right panel). c Relative CDKN1A expression in SDS patient-derived fibroblasts transduced with either shLUC control or shEIF6 (left panel) and shTP53 (right panel). Error bars represent mean ± standard deviation of three technical replicates representative of two to three independent experiments. p value calculated using unpaired two-tailed t-test.

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