Fig. 1: EJC core components, eIF4A3 and Y14, strongly localize around centrosomes in quiescent mNSC, but not in differentiated ependymal cells.

Quiescent mNSC (mouse neural stem cell; a, c) and multiciliated ependymal cells (b, d) were stained for eIF4A3 (a, b) or Y14 (c, d). Centrosomes were labeled by FOP antibody. Primary cilia and centriole were stained by poly-glutamylated tubulin (PolyGlu-Tub) antibody. Nuclei were stained by Hoechst. Images result from maximum intensity projections of 12 z-stacks acquired at every 0.5 μm. Lower panels show enlarged images that are marked by white dashed square in the upper panel. Scale bars in the upper and lower panels are 5 and 3 μm, respectively (a–d). Yellow arrows depict concentrated EJC proteins (a, c). Fluorescence intensities for eIF4A3 (e) and for Y14 (h) were quantified in 2 μm circles around centrosomes and plotted as fluorescence intensities relative to the average fluorescence intensity in quiescent mNSC (set as 1.0). The red lines mark the median values and values between the 25th lower percentile and 75th higher percentile are in the box. Whiskers above and below the box correspond to 0.35th lower percentile and 99.65th higher percentile, respectively (e, g). The fraction of cells with detectable centrosomal eIF4A3 (f) or Y14 (h) was determined in either quiescent mNSC or ependymal cells. Data are presented by mean + SD of three independent experiments (f, h). ****P ≤ 0.0001, two-tailed Mann–Whitney test (e, g) and two-tailed t-test (f, h). The number of cells analyzed in three independent experiments is provided (e–h). Source data are provided as a Source Data file.