Fig. 4: Immunogenicity of S-Trimer in nonhuman primates. | Nature Communications

Fig. 4: Immunogenicity of S-Trimer in nonhuman primates.

From: S-Trimer, a COVID-19 subunit vaccine candidate, induces protective immunity in nonhuman primates

Fig. 4: Immunogenicity of S-Trimer in nonhuman primates.The alternative text for this image may have been generated using AI.

Rhesus macaques (n = 6/group) were immunized with 30 µg S-Trimer adjuvanted with 0.25 mL AS03, 30 µg S-Trimer adjuvanted with 1.5 mg CpG 1018 plus 0.75 mg alum, or PBS vehicle control twice on Day 0 and Day 21 and were challenged on Day 35 with 2.6 × 106 TCID50 (60% intranasal and 40% intratracheal) live SARS-CoV-2 virus. The humoral immune responses and kinetics were analyzed and compared with a human convalescent sera (HCS) panel, based on a S-Trimer binding antibody ELISA titers, b ACE2-competitive ELISA titers, c SARS-CoV-2 pseudovirus neutralization titers, and d wild-type SARS-CoV-2 virus neutralization titers. Circular points represent individual animals and humans; kinetics data are presented as geometric mean titers (GMT) ± SEM. For statistical analysis of antibody titers, all comparisons were made against the vehicle negative control group with Two-way ANOVA multiple comparisons test. P values < 0.05 were considered significant.

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