Fig. 5: TZP retraction requires ERK activity. | Nature Communications

Fig. 5: TZP retraction requires ERK activity.

From: Epidermal growth factor receptor signaling uncouples germ cells from the somatic follicular compartment at ovulation

Fig. 5

a Confocal images of COCs obtained from antral follicles of eCG-primed mice and incubated under the indicated conditions. Dashed circle outlines oocyte nucleus. Inset in the U0126 image shows condensed chromosomes, confirming that GVBD has occurred. b Mean and SEM of number of TZPs following incubation under the indicated conditions, normalized to the number at 0 h. Each point represents an individual COC. n = 19 (0 h), 38 (8 h EGF), 28 (8 h EGF U0126) COCs examined over three independent experiments. Statistical analysis using one-way ANOVA with Tukey multiple-comparison test. **** P < 0.0001. c Following an 8 h exposure of COCs to EGF+/− U0126, Lucifer Yellow was injected into the oocyte and fluorescence was visualized 30 min later. Inset is an over-exposure to confirm that cumulus cells are present in the control group. Scale bar = 10 µm (a); 20 µm (c).

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