Fig. 6: DREADDs modulate intracellular calcium of immature adult-born granule cells.

a Sham experimental timeline of injections and slice preparation. b Representative images of hM3Dq-mCherry cell co-expressing GCaMP6f. DIC image of abGCs of the dentate gyrus in slice preparation. Scale bar represents 50 µm. c Example traces of the change in fluorescence over time from all cells in the dataset (n = 10 cells). Baseline (light gray) is displayed over the first 60 s, followed by 60 s of CNO response (red), and 60 s of ACSF washout (dark gray). Quantification of d event frequency, e event amplitude, f area under the curve (AUC). g Representative image of hM4Di-mCherry expression in 2-week-old aberrant abGCs. h Time lapse representation for 2-week-old abGC. Scale bar represents 10 µm. i Example traces of the change in fluorescence over time of all cells in the dataset (n = 10 cells). Quantification of j event frequency, k event amplitude, l area under the curve (AUC) for aberrant abGCs. Data presented as mean ± SEM. n = 4 mice per Sham group (d, e) and 3 mice per Pilo group (j–l). *p < 0.05, using ANOVA with repeated measures. All statistics calculated using two-tailed test.