Fig. 1: Characteristics of pHmScarlet.
From: pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps
a Excitation spectra and b Absorbance spectra of pHmScarlet at different pH values. c pH titration curves of pHmScarlet (red) and pHuji (black). d Excitation and emission spectra of pHmScarlet (red) and pHuji (black) at pH 7.4. e SNAP-normalized fluorescence of FPs in U-2 OS cells expressing H2B-pHmScarlet or H2B-pHuji (pHuji: 0.32 ± 0.05, pHmScarlet: 2.27 ± 0.64, p = 0.0007, two-sided Student’s t tests. Data are presented as mean ± SD). f Photobleaching curves of pHmScarlet (red) and pHuji (black). g t1/2 of pHmScarlet and pHuji (pHuji: 1.1 ± 0.1, pHmScarlet: 28.3 ± 2.5, p = 0.007, two-sided Student’s t tests. Data are presented as mean ± SD). Results shown are from three independent experiments. **p <0.01, ***p <0.001. Source data is provided as a source data file.
