Fig. 5: Detection of the docking stage in INS-1 cells.
From: pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps
Representative kymograph and the corresponding time-lapse fluorescence-intensity curves showing the distinct kinetic properties of a docking-free, b short docking, and c long docking exocytotic events. d Distribution of docking dwell times (total exocytotic evens, n = 138; three cells were examined over two independent experiments). Representative kymograph and corresponding time-lapse fluorescence-intensity curves showing the exocytosis of the same vesicle co-labeled with e VAMP2-pHmScarlet (magenta) and VAMP2-SEP (green), f VAMP2-pHmScarlet (magenta) and VAMP2-EGFP (green), g VAMP2-pHuji (magenta) and VAMP2-SEP (green), or h VAMP2-pHmScarlet (magenta) and VAMP2-EGFP (green). Source data is provided as a source data file.
