Fig. 5: Cryo-ET structure of VPS34 complex II on Rab5a–GTP-coated membranes.
From: Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes
a Section through a cryo-electron tomogram of Rab5a-decorated vesicles coated with VPS34 complex II–BATS. Scale bar 100 nm. b Subtomogram averaging reconstruction of human complex II–BATS bound to membrane-attached Rab5a–GTP at 9.8 Å with the membrane masked-out. A model of human complex II based on the crystal structure of yeast complex II (5DFZ) was fit to the density and refined with restraints. c Rab5a (PDB entry 3MJH) was optimally fit into the cryo-ET density. In this orientation, the Rab5a switch 2 helix faces the VPS34 C2 insertion (C2HH). The Rab5a density is nestled between the C2HH, the VPS15 SGD and WD40 domains. d The crystal structure of the inactive state of yeast complex II, in which the VPS15 kinase domain (N terminus coloured pink) contacts and inhibits the VPS34 kinase domain (activation loop coloured green). e The cryo-ET structure of the active form of human complex II bound to membrane-attached Rab5a–GTP in the same orientation as d.
