Fig. 8: KIF17 is involved in ATF4-dependent TRPM3 membrane trafficking.
From: ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking
a Colocalization of the KIF17, ATF4 and TRPM3 proteins in mouse DRG sections. Scale bar, 200 μm. b Colocalization of KIF17 mRNA, ATF4 mRNA and TRPM3 mRNA in mouse DRG sections. Scale bar, 20 μm. c Colocalization of the KIF17, ATF4 and TRPM3 proteins in DRG neurons was detected by SIM. Scale bar, 5 μm. d, e Changes in the membrane expression of TRPM3 in the DRG after KIF17 knockdown (d) or KIF17 overexpression (e). n = 6 mice per group. F(2,15) = 34.36, P = 0.0002 in d. F(2,15) = 20.48, P = 0.0005 in e. f The behaviours of KIF17 siRNA- and scrambled siRNA-injected mice were evaluated by the Hargreaves test. n = 6 mice per group. t10 = 3.485, P = 0.0059. g The behaviours of KIF17-overexpressing and control mice were evaluated by the Hargreaves test. n = 6 mice per group. t10 = 6.776, P < 0.0001. h ATF4 knockdown suppressed the increased expression of TRPM3 in the membrane induced by KIF17 overexpression. n = 6 mice per group. F(2,15) = 16.19, P = 0.0002 in naïve vs. KIF17-over, P = 0.0014 in KIF17-over vs. KIF17-over + ATF4-siRNA. i KIF17 knockdown inhibited the increase in TRPM3 expression on the cell surface induced by ATF4 overexpression. n = 6 mice per group. F(2,15) = 12.76, P = 0.0025 in naïve vs. ATF4-over, P = 0.0010 in ATF4-over vs. ATF4-over + KIF17-siRNA. **P < 0.01. d, e, h, i One-way ANOVA followed by Tukey’s multiple comparisons test. f, g Two-tailed Independent Student’s t test. The error bars indicate the SEMs.
