Fig. 3: Evidence for tweezer binding to Survivin120 from ITC measurements.
From: Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
Titration of 300 μM TW (a) in the cell with 300 µM Survivin120 in the syringe. Titration of 100 µM TW-ELTL (b) and TW-ELTLGEFL (c) in the cell with 1.2 mM Survivin120 in the syringe. All titrations were performed in PBS, pH 7.4 at 25 °C. The black lines in the bottom panels are the best fit of the data to a one set of sites model. The heat of dilution was subtracted as constant. Dissociation constants were determined to be 38 ± 4 µM for TW, 24 ± 4 µM for TW-ELTL and 19 ± 3 µM for TW-ELTLGEFL. Values reported are the mean ± SEM of the fit. For thermodynamic parameters see SI7. Source data are provided as a Source Data file.
