Fig. 4: sGD driving experiments in cage trials.
From: Inherently confinable split-drive systems in Drosophila
Virgin sGD/Cas9 trans-heterozygotes and WT flies were seeded at a 1:3 ratio. Each generation, flies in a cage were randomly split in half. One half was scored for eye fluorescence (Gn) while the other was used to seed fresh cages (Gn+1). Purple traces indicate sGD+ progeny, green traces indicate Cas9+ progeny. Experiments were done in triplicate and each line represents a separate cage. a rab5 sGD; vCas9-III. The sGD prevalence increases exponentially in the cage (84±6%) up to G4 and then plateaus slowly. sGD highest percentage occurs at G10 (92 ± 4%). Cas9 decreases in two of the three cages from 25% to 0% by G8 and G15, respectively. b spo11 sGD; vCas9-III. All three replicates reach their highest prevalence in the cage (85 ± 2%) by G6-7 and then plateau. Cas9 decreases linearly from 25% to 0% by G15 in all three replicates. c rab11 sGD; vCas9-X. sGD proportions in the cage slowly increase linearly (83±6%) up to G8. Cas9 remains steady at seeding levels (28 ± 3%), suggesting continuous Mendelian transmission. d prosalpha2 sGD drive dynamics depend on the location of the static Cas9, as well as seeding ratios. Bold lines reflect sGD; vCas9-X, thin dashes show cage trials using vCas9-III and thicker dashes depict drive in a vCas9-X-saturated population. Prosalpha2 sGD; vCas9 combinations produce different driving fates and outcomes, providing a flexible tool for deployment. e Hypothesis on cage and drive behavior of the different sGDs and vCas9 reduction over time. Raw phenotypic scoring is provided as “Supplementary Data 2”.
