Fig. 5: Single-cell V(D)J sequencing reveals the clonal expansion and activation-to-exhaustion transition in exhausted and immunosuppressive T cell subtypes. | Nature Communications

Fig. 5: Single-cell V(D)J sequencing reveals the clonal expansion and activation-to-exhaustion transition in exhausted and immunosuppressive T cell subtypes.

From: Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma

Fig. 5

a Distribution of T cell clones by size, with different Shannon index showing the clonal diversity for each patient. b The relative abundance of different clone sizes in the NPC-derived (n = 7 biologically independent samples) and NLH-derived (n = 3 biologically independent samples) T cells. Each dot represents one patient. The two-sided Student’s t test was used to determine the statistical significance, p values ≤ 0.05 were represented as *, ≤0.01 as **, ≤0.001 as ***, ≤0.0001 as ****. Data are presented as mean values ± SD. c The Spearman’s correlation between the calculated exhaustion score/Treg score and the fraction of clones with ≥3 cells in patients. Source data are provided as a Source Data file. d UMAP plot of the top three largest clones in patients 4, 9 (NPC) and 12, 13 (NLH), with their associated clonotypes. (e and f) The developmental trajectory of CD8+ and CD4+ T cells, colored-coded by the associated cell subpopulations and numbered by different developmental branches.

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