Fig. 1: Single-cell sequencing reveals major differences between adolescent and adult preadipocytes.
From: Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population
Single-cell sequencing of cells of the subcutaneous stromal vascular fraction from 2- to 8-week-old male C57Bl/6 mice. Cells were pooled from the SCF of seven (2 weeks old) and five (8 weeks old) mice. T-stochastic neighborhood embedding (t-SNE) of union of adolescent and adult cells of the SCF SVF (n = 9344) with mouse age (a) and Louvain group (b) superimposed. The circle indicates clusters containing preadipocytes. c Log1p expression [log1p = log(X + 1), where X is the expression of gene of interest in particular cell. Note that X, the mRNA counts from the alignment, are integer numbers] of different cell type markers in the 11 louvain clusters by cell (n0 = 1733, n1 = 1379, n2 = 1222, n3 = 948, n4 = 892, n5 = 816, n6 = 799, n7 = 518, n8 = 514, n9 = 421, n10 = 102). Leukocytes (Ptprc), T cells (CD3d, CD3g), NK cells (Nkg7, Klrd1), myeloid cells (Fcgr3, S100a8), macrophages (Adgre1, Lyz2), dendritic cells (CD74, CD83), B cells (CD19), megakaryocytes (Ppbp), erythrocytes (Gypa), and preadipocytes (Pdgfra, Fbn1, Col4a1, CD34, CD24a, Dlk1, Asc-1). d t-SNE of union of adolescent and adult cells of the SCF SVF (n = 9344) with log1p Pdgfra and Asc-1 expression superimposed. e t-SNE of preadipocytes from adolescent and adult SCF (n = 3,062) colored by age. f Volcano plot of comparison between adolescent and adult preadipocytes with differential expression (false discovery rate-corrected P value <0.01, Welch’s t test) indicated by log1p expression. Each dot is a gene. Black: not differentially expressed, yellow: differentially expressed, red: Asc-1. g t-SNE of preadipocytes from adolescent and adult SCF (n = 3062) colored by Asc-1 expression.
