Fig. 6: Chemical ligation of oligodeoxynucleotides and single-step synthesis of photocaged oligonucleotides. | Nature Communications

Fig. 6: Chemical ligation of oligodeoxynucleotides and single-step synthesis of photocaged oligonucleotides.

From: Programmable site-selective labeling of oligonucleotides based on carbene catalysis

Fig. 6

a Synthetic scheme for the ligation of oligonucleotides with an oxime ether linker. b Denaturing PAGE analysis of the ligation visualized by SYBR gold. Lanes; L: DNA ladder, 1: before acetonylation, 2: after acetonylation, 3: after ligation, 4: 12a, 5: 12b, 6: 12c. c Synthesis of photocaged single-stranded oligoribonucleotide based on the G-bulge protocol. d Caging and decaging of 17b. Source data are provided as a Source Data file.

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