Fig. 2: SLC7A11 modulates ferroptosis sensitivity to class 2 FINs partly through regulating GPX4 levels.
From: mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation
a UMRC6 cells were treated with RSL3 or ML162 at indicated concentrations for 10 h followed by cell viability analysis. b UMRC6 cells were treated with 600 nM RSL3 combined with or without 5 μM Ferrostatin-1 (Ferr-1) or 100 μM deferoxamine (DFO) for 6 h. Then lipid peroxidation was assessed using BODIPY™ 581/591 C11 staining followed by FACS analysis. c Cell viability was determined for control (sgCon) and SLC7A11-knockout (sgSLC-1 and sgSLC-2) UMRC6 cells treated with 500 nM RSL3 combined with or without 5 μM Ferrostatin-1 (Ferr-1) or 100 μM deferoxamine (DFO) for 10 h. n = 4. d Empty vector (EV) and SLC7A11-overexpressing (SLC7A11) 786-O cells were treated with RSL3 or ML162 at indicated concentrations for 9 h followed by cell viability analysis. e Representative photos of 786-O-EV and -SLC7A11 cells treated with 400 nM RSL3 for 9 h. f 786-O-EV and -SLC7A11 cells treated with 400 nM RSL3 for 6 h. Then lipid peroxidation was assessed using BODIPY™ 581/591 C11 staining followed by FACS analysis. g UMRC6 cells were treated with RSL3 or ML162 at indicated concentrations in the presence of 5 μM erastin or 1 μM cystine (Cystine low) for 10 h followed by cell viability analysis. h Western blotting analysis of GPX4 expression in H1299-EV, -SLC7A11, and H1299-SLC7A11 GPX4-knockdown cell lines. SE short exposure, LE long exposure. i Cell viability measured in cell lines described in h treated with indicated concentrations of RSL3 for 6 h. j Western blotting analysis of GPX4 expression in control (sgCon) and SLC7A11-KO (sgSLC-1 and sgSLC-2) UMRC6 cell lines overexpressed with empty vector (EV) or GPX4. k, l Cell viability measured in cell lines described in j treated with indicated concentrations of RSL3 for 10 h. n = 3. For all panels, error bars are mean ± SD. n indicates biologically independent repeats. P value was determined by two-tailed unpaired Student’s t test. For i, p value was determined by two-way ANOVA test. Source data are provided as a Source Data file.
