Fig. 3: Spatial RNA-seq identifies spatially restricted genes in cardiac tissue during development. | Nature Communications

Fig. 3: Spatial RNA-seq identifies spatially restricted genes in cardiac tissue during development.

From: Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis

Fig. 3

a Spatial RNA-seq barcoded spots clustered by gene expression and labeled by tissue anatomical compartment for four developmental stages. b Average cell type composition across various tissue anatomical compartments. c Spatial map showing the cell type heterogeneity for every spot in cardiac tissue across stages. Cell type was estimated by enumerating the number of distinct cell-types with prediction scores greater than 5%. d–h Spatially resolved gene expression for spatially restricted genes. d TBX5 overexpressed in left ventricles on day 7 and day 10. e TBX20 showing no spatial restriction. f CHGB overexpressed in right ventricles across stages. g ACTG2 overexpressed in right ventricles on day 7. h MB expressed in compact myocardium on day 10 and day 14. MB expression increases with the developmental stage. i smFISH stained day 7 and day 14 hearts for TBX5 (red), TBX20 (orange), TNNT2 (green), and DAPI (blue) confirming TBX5 overexpressed in left ventricles of day 7 hearts but not day 14 and TBX20 showing no left or right ventricle expression differences. Representative images of three to four biological replicates.

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