Fig. 3: BAF co-localizes with LDTFs and MLL4 on active enhancers during adipogenesis.

a Average binding profiles of SMARCA4, SS18, and ARID1A around the center of SMARCA4⁺ active enhancers (AEs) or promoters before (D-3) and during (D2) adipogenesis of brown preadipocytes. Normalized read counts are shown. b Motif analysis of the top 3,000 SMARCA4⁺ SS18⁺ AEs using SeqPos motif tool. c Pie chart depicting BAF-binding (SMARCA4⁺ SS18⁺) AEs at D2 of adipogenesis. Among the 10,813 BAF-binding AEs at D2, 6,403 are de novo BAF-binding AEs (BAF-de novo), while 4,410 AEs are already bound by BAF (BAF-prebound) at D-3. d, e BAF subunits SMARCA4, SS18 and ARID1A colocalize with LDTFs (C/EBPβ, C/EBPα, and PPARγ), MLL4, CBP and open chromatin on AEs (H3K4me1⁺ H3K27ac⁺) at D2 of adipogenesis. The local epigenetic environment exhibits coordinated changes during differentiation. Heat maps (d) and average profiles (e) were aligned around the center of SMARCA4⁺ SS18⁺ AEs. Published ChIP-Seq data sets for MLL4, CBP, C/EBPβ, C/EBPα, and PPARγ were used (GSE74189)¹⁸.