Fig. 4: UTX and MLL4 associate with SWI/SNF complex subunits in cells.
From: Interplay of BAF and MLL4 promotes cell type-specific enhancer activation
a UTX and MLL4 associate with SWI/SNF components in mouse embryonic stem cells. Nuclear extracts were subjected to immunoprecipitation with anti-UTX or anti-MLL4 antibodies. Components of MLL3/4 and SWI/SNF complexes identified by mass spectrometry after immunoprecipitation of endogenous UTX or MLL4 are presented (n = 1). Unique and total peptide numbers for IP-enriched BAF and PBAF complex subunits as well as MLL4 complex subunits are shown. Full lists of identified proteins are presented in the Supplementary Data 1 and 2. b, c MLL4 complex associates with SMARCA4 and SMARCB1 in cells. Nuclear extracts from HEK293T cells (b) or preadipocytes during (D2) adipogenesis (c) were subjected to immunoprecipitation with anti-MLL4 or anti-UTX antibody. Immunoprecipitates were analyzed by Western blotting with antibodies indicated on the left. n = 2 biological replicates.
