Fig. 2: Adeno-associated virus (AAV) capsid structure and heterogeneity.
From: Adeno-associated virus capsid assembly is divergent and stochastic
a Coomassie stained SDS-PAGE and negative stain EM images of VP3-only AAV1 (left) and wild-type AAV1 (right) capsids. b Native MS spectrum of the VP3-only AAV1 capsids, with an assigned mass of 3,571 ± 0.3 kDa (masses are reported as mean ± s.d.), is shown at the top. The simulated native spectrum for capsids with a 0:0:60 (#VP1:#VP2:#VP3) stoichiometry is shown below and accounts for all observed peaks in the experimental data. c Native MS spectrum of wild-type AAV1 capsids, showing three peak series with a relative mass shift of around 6.5 kDa. The simulation of several capsid stoichiometries are shown below and illustrate the potential coinciding charge state distributions caused by VP3-to-VP2 and VP3-to-VP1 substitutions. VP ratios are indicated for each simulation respectively (#VP1:#VP2:#VP3).
