Fig. 3: Stochastic assembly model for complete AAV capsids.
From: Adeno-associated virus capsid assembly is divergent and stochastic
a Illustration of AAV capsid assembly following a stochastic model, where VPs are drawn randomly from the pool of expressed VPs. The probability for each capsid stoichiometry is solely determined by the ratio of the expressed VPs. Here displayed for AAV9 and the experimentally determined expression levels of 6% VP1, 16% VP2, and 78% VP3 or approximately 3:10:47 (VP1:VP2:VP3). The probabilities for exemplary VP stoichiometries are indicated above each capsid, respectively. b The calculated probabilities for all possible 1891 capsid stoichiometries are displayed as a heat map. The number of VP1 and VP2 in the 60-subunit capsids are indicated on the plot axis. c Summed probabilities for capsids having a specific number of VP1, VP2, or VP3 in their capsid.
