Fig. 4: ESI and native MS of complex AAV samples.
From: Adeno-associated virus capsid assembly is divergent and stochastic
a Theoretical mass distribution for all possible capsid stoichiometries of AAV9 and the resulting theoretical mass spectrum at infinite resolution. The mass distribution shows the 1,891 possible capsid masses with their probability as calculated in Fig. 2b. After ESI, each mass is simulated with 37 charge states yielding a total of 69,967 different ion species. b, c Simulated mass spectra for AAV9, showing the convoluted ion signals of the 69,967 different ion species shown in a) at resolutions, corresponding to transient times of 32 ms and 128 ms. Each plot shows (top to bottom) the simulated mass spectra before and after baseline correction with their experimental counterpart. Experimental data was recorded by transient averaging, thereby removing the unresolved regions presented in the non-baseline corrected. d Comparison (top to bottom) between 32 ms and 128 ms mass spectra and simulation at infinite resolution (data points/FT bins are shown as dots).
