Fig. 5: Top scoring optimized simulations for several distinct AAV products of different serotypes.
From: Adeno-associated virus capsid assembly is divergent and stochastic
a–f Scores for the systematic comparison between simulations and experimental mass spectra are displayed for AAV1 VP3-only (a) and the wild-type capsids for AAV1 (b), AAV8_1 (c) and AAV8_2 (d), AAV5 (e), and AAV9 (f). AAV8_1 and AAV8_2 are different batches produced in different laboratories. Simulated mass spectra for the best scoring bulk VP ratios are shown with the experimental data alongside the corresponding probability histograms for capsids having a certain number of VP1, VP2, or VP3. Best scoring bulk VP ratios for each serotype are listed in Table 1. The amount of capsids missing either a VP1 or VP2 are indicated above the histograms and range between 100% for AAV1 VP3 only (a) and 2% for AAV9 (f).
