Fig. 2: KIV reamination across tissues in living rats.
From: Branched-chain α-ketoacids are preferentially reaminated and activate protein synthesis in the heart
Thirty minutes after a single injection of BCAT inhibitor, LY3351337 (10 mg/kg i.p.; n = 5; blue), or Veh (DMSO; n = 5; gray), Wistar rats received an i.p. injection of [U-13C]KIV (100 mg/kg), followed by blood sampling from the tail vein at 2, 5, 10, 15, 30, and 60 min. Fractional percent labeling with 13C of (a) plasma KIV and (b) plasma valine. N = 5–6 per group. A separate cohort of Wistar rats was treated with LY3351337 (10 mg/kg i.p.) or vehicle and received an i.p. injection of [U-13C]KIV (100 mg/kg) 30 min later. Tissues were harvested 30 min after the [U-13C]KIV injection. c Fractional percent labeling with 13C of valine in plasma and the indicated tissues in the absence (Veh; n = 8) or presence of LY3351337 (n = 10). The dashed line indicates the plasma enrichment level. Data represent mean ± SEM. Statistical differences indicated by a two-way paired Student’s t-test: *P < 0.05, **P < 0.005.
