Fig. 3: Over-expression of SLC25A44 decreases KIV reamination in the isolated perfused mouse heart.
From: Branched-chain α-ketoacids are preferentially reaminated and activate protein synthesis in the heart
a Correlation of 13C-valine concentration with Bcat1, Bcat2, and Slc25a44 mRNA levels across rat heart (n = 6; red), liver (n = 5; blue), kidney (n = 6; green), and gastrocnemius (n = 5; orange). Data for tissue labeling of 13C valine was obtained in rats injected with [U-13C] KIV, as summarized in Fig. 2C. b Experimental design for the study of AAV-mediated overexpression of SLC25A44 in mouse heart. c Relative mRNA expression of Slc25a44 in isolated perfused hearts following treatment with AAV9-CMV-SLC25A44 (n = 7; purple) versus AAV9-CMV-GFP (n = 7; navy). The concentration of 13C-labeled valine in the heart (d), the total rate of formation of 13C-labeled valine (e), perfusate concentration of 13C-labeled valine (f), and concentrations of 13C-labeled TCA cycle intermediates from [U-13C]KIV (100 μM) (g) following treatment with AAV9-CMV-SLC25A44 or AAV9-CMV-GFP. h Relative levels of transcripts encoding BCAA transporters in AAV9-CMV-SLC25A44 versus AAV9-CMV-GFP-treated hearts N = 7 per group. i Correlations of Scl25a44 with Slc7a5 mRNA levels in rat heart, liver, kidney, or gastrocneminus (gastroc) muscle. N = 5–6 per group. Data represent mean ± SEM. Statistical differences indicated by Pearson correlation (a, i) or a two-way, paired Student’s t-test (c–h): *P < 0.05, ***P < 0.0005.
