Fig. 4: RNA-seq profiling revealed that PRMT4, 5, and 7 exhibited a global impact on RNA alternative splicing.

a HEK293 cells transfected with siCTL or siPRMT4, siPRMT5 or siPRMT7 were subjected to RNA-seq analysis, and the number of alternative splicing events regulated is shown (|ΔPSI| ≥ 0.5). Cassette exons (SE); intron retain (IR); mutually exclusive exons (MXE); alternative 5′ splice sites (5′ ASS) and alternative 3′ splice sites (3′ ASS). b The number of exon inclusion (EI) and skipping (ES) induced is shown. c All alternative splicing events regulated by PRMT4, 5, and 7 were classified into three categories, events regulated by one (light green), any two out of three (light black) or all three (light purple) PRMTs. d Overlap between EI and ES induced by PRMT4, 5, or 7 is shown. e Overlap among PRMT4-, 5-, and 7-regulated cassette exons is shown. f HEK293 cells transfected with siCTL or two individual siPRMT4 (siPRMT4-1 and siPRMT4-2), siPRMT5 (siPRMT5-1 and siPRMT5-2), or siPRMT7 (siPRMT7-1 and siPRMT7-2) were subjected to analysis of the expression of both short and long isoforms of representative genes as indicated. The length of the alternatively spliced exon, as well as the expected length of the PCR products, was shown as indicated. DNA marker (M) was included on the left (bp: base pair). The position of the cassette exon in each gene was as follows: PPARA (NM_005036, exon3); SREBF2 (NR_103834, exon18); RAB27B (NM_001375327, exon3); WDPCP (NM_015910, exon6). F: forward primer; R: reverse primer. The translation start sites (ATG) of the two isoforms of RAB27B were indicated by asterisks. n = 3 biological replicates and representative data are shown. g–i HEK293 cells transfected with siCTL or siPRMT4 (g), siPRMT5 (h), or siPRMT7 (i) in the presence or absence of wild-type (WT) or enzymatic dead mutant (MT) PRMT4 (g), PRMT5 (h), or PRMT7 (i) were subjected to alternative splicing analysis as described in (f). Source data are provided as a Source data file.