Fig. 7: PRMT4, 5, 7, and hnRNPA1 arginine methylation were required for the growth of multiple types of cancer cells. | Nature Communications

Fig. 7: PRMT4, 5, 7, and hnRNPA1 arginine methylation were required for the growth of multiple types of cancer cells.

From: Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth

Fig. 7: PRMT4, 5, 7, and hnRNPA1 arginine methylation were required for the growth of multiple types of cancer cells.

a–c, f MCF7 cells were transfected with siCTL or siPRMT4, 5, or 7 followed by cell proliferation assay (a–c) and alternative splicing analysis (f). n = 3 biological replicates for cell proliferation assay (mean ± SD, ***P < 0.001, day 4 by unpaired Student t-test, two-tailed). n = 3 biological replicates for alternative splicing analysis and representative data are shown. d, g MCF7 cells were transfected with siCTL or sihnRNPA1 followed by cell proliferation assay (d) or alternative splicing analysis (g). n = 3 biological replicates for cell proliferation assay (mean ± SD, ***P < 0.001, day 4 by unpaired Student t-test, two-tailed). e, h MCF7 cells transfected with siCTL or sihnRNPA1 in the presence or absence of wild type (WT) or methylation deficient mutants including R/K (4), R/K (5), R/K (7), and R/K (457) were subjected to cell proliferation assay (e) or alternative splicing analysis (h). n = 3 biological replicates for cell proliferation assay (mean ± SD, ***P < 0.001, day 4 by unpaired Student t-test, two-tailed). i, j MCF7 cells were transfected with siCTL or siPRMT4, siPRMT5, siPRMT7, or sihnRNPA1 in the presence or absence of Flag-tagged WDPCP (i) or RAB27B (j), both short (S) and long (L) isoforms, followed by cell proliferation assay. n = 3 biological replicates (mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, N.S: non-significant, day 4 by unpaired Student t-test, two-tailed). Source data are provided as a Source data file.

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