Fig. 6: The US9 Ig-like and transmembrane domains, but not the US9 SP, bind MICA*008, and the ERAD scaffold protein SEL1L.
From: A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain
a Lysates prepared from RKO MICA*008-HA cells co-transduced with EV or US9 were immunoprecipitated using anti-His tag antibody. Immunoblotting was performed using anti-His tag, anti-MICA, and anti-SEL1L to visualize protein co-precipitation, with anti-vinculin as input loading control. Arrows indicate bands which specifically co-precipitated with US9 (red—SEL1L; black—MICA*008). * antibody light chain. Representative of three independent experiments. b Lysates prepared from the indicated RKO MICA*008-HA transfectants were immunoprecipitated using anti-His tag (A—input; B—IP) and immunoblotted using anti-His tag, anti-MICA, and anti-SEL1L. Anti-vinculin served as input loading control. * antibody light chain. Representative of two independent experiments. ND not detected. See also Fig. S6 and Supplementary Data 6.
