Fig. 8: US9 SP arrests MICA*008 maturation in SEL1L’s absence.
From: A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain
RKO MICA*008-HA cells expressing shScrambled (shSCR) or shSEL1L#2 (sh#2) were co-transduced the indicated constructs. a Quantification of MICA*008 surface expression on the indicated cells measured by flow cytometry. MFIs were normalized to the MFI of shSCR EV transfectants. Data show mean ± SEM for at least three independent experiments per construct. A two-way ANOVA (knockdown, US9 construct expression) was conducted to compare the normalized MFIs of the various constructs in shSCR vs. shSEL1L cells. The main effect of knockdown was significant at the p < 0.05 level [F(1,56) = 41.46, p = 2.01 × 10−8]. The main effect of US9 construct expression was also significant [F(4,56) = 276.73, p = 3.59 × 10−36]. There was a significant interaction between knockdown and US9 construct expression [F(4,56) = 20.16, p = 2.5 × 10−10]. A post hoc Tukey test was used to compare EV MFI (dashed line) to that of each construct in the shSEL1L-expressing cells. **p < 0.01. b Lysates were obtained from the indicated cells and blotted using anti-MICA and anti-vinculin loading control. The red arrow indicates glycosylation-trimmed MICA*008. Representative of two independent repeats. See also Figs. S7–8 and the Source Data file for experimental data and full statistics.
