Table 1 Characteristics and function of US9 mutants.

Construct name	Mutated domain	US9 SP status	Effect on MICA*008^a	Related figure
US9	−	Slowly cleaved	++	Fig. 3
ΔCyto+TM	Cytosolic and transmembrane	Slowly cleaved	++
Q214A	Transmembrane	Slowly cleaved	++
8TM	Transmembrane	Slowly cleaved	++
ΔC-Lum	Luminal	Slowly cleaved	++
ΔIg	Luminal	Uncleaved	++
ΔSer	Luminal	Uncleaved	++
ΔN-Ser	N-terminal	Rapidly cleaved	+
8SP	N-terminal	Swapped	+
8SPΔCyto	N-terminal, Cytosolic	Swapped	+	Fig. 5
8SPΔCyto+TM	N-terminal, Cytosolic and transmembrane	Swapped	−
8SPQ214A	N-terminal, transmembrane	Swapped	+
8SP8TM	N-terminal, transmembrane	Swapped	+
8SPΔC-Lum	N-terminal, luminal	Swapped	+
8SPΔIg	N-terminal, luminal	Swapped	−
8SPΔSer	N-terminal, luminal	Swapped	+
8SPΔN-Ser	N-terminal	Swapped	+

^a++: MICA*008 surface expression <10% of empty-vector (EV) control; +: MICA*008 surface expression 10–75% of EV control; −: MICA*008 surface expression >75% of EV control.

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