Fig. 3: Snx9-dependent MDVs contribute to the inclusion of IM/matrix proteins into EVs. | Nature Communications

Fig. 3: Snx9-dependent MDVs contribute to the inclusion of IM/matrix proteins into EVs.

From: Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs

Fig. 3

a Schematic representation of the two distinct MDV pathways leading to EVs and lysosomes, respectively. b MEFs were treated for 24 h with a control siRNA (siCtrl, blue) or a siRNA against Snx9 (siSnx9, red). Mitochondrial proteins were then measured by western blot, with Actin serving as a loading control. c Representative images showing TOM20 and mtHSP70 MDVs in MEFs treated with a control siRNA (siCtrl) or a siRNA against Snx9 (siSnx9). Arrowheads denote MDVs (positive for one marker but negative for the other). Scale bars: top panels, 10 µm; enlargements in bottom panels, 2 µm. d MDV quantification from images as in (c). Each data point represents one cell. Bars represent the average of 40 cells in three independent experiments ± SD. Two-sided t-test. e Enrichment of the indicated mitochondrial proteins was measured by western blot as in Fig. 1. Individual points represent independent experiments (n = 4). Bars are shown as average ± SD. Two-sided t-test.

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