Fig. 1: Diet and cystathionine γ-lyase (CGL) status impact H₂S production in a tissue specific manner.

a Graphical presentation of the overarching experimental setup. Six-month-old male CGL wildtype (WT) and total body knock out (KO) mice were placed on ad libitum (AL) or 50% dietary restriction (DR) diets for 1 week prior to tissue harvest. Tissues were analyzed for (i) H₂S production capacity via the lead acetate/lead sulfide method, (ii) protein persulfidation profiles via the biotin thiol (BTA) assay, and (iii) biological pathway enrichment/function of the identified persulfidated proteins. b, c H₂S production capacity in tissues in AL fed CGL WT (n = 3 mice/group) and CGL KO (n = 3 mice/group) mice at short 6 h (b) and long 16 h (c) exposures of the same assay plate. Means are shown and error bars are ± SEM. The asterisk indicates the significance of the difference in the average tissue-specific H₂S production capacity between CGL WT and KO mice after subtracting the (–) Tissue negative controls as background; with P values obtained from two-sided Student’s t test. d–i H₂S production capacity in tissues harvested from CGL WT mice after 1 week of AL (n = 4 mice/group) or 50% DR (n = 5 mice/group) feeding. j, k H₂S production capacity in liver (j) and kidney (k) in CGL KO mice after 1 week of AL (n = 3 mice/group) or 50% DR (n = 3 mice/group) feeding. Means are shown and error bars are ± SEM. The asterisks indicate the significance of the difference between the dietary treatment groups; with P values obtained from two-sided Student’s t test. ns not significant. Negative values after subtracting background have been converted to zero. The dash mark in the lead acetate/lead sulfide images in (d–i) indicates no sample loaded in that location. Source data are provided as a Source Data file. See also Supplementary Fig. 1.