Fig. 4: Effect of Ifnar1^SA on mRNA expression gene expression by PMN-MDSCs.

a mRNA expression of genes associated with immunosuppressive activity in PMN-MDSCs. Analyzed by RT-qPCR. b Reactive oxygen species (ROS) levels by PMN-MDSCs from spleen and tumors of WT and Ifnar1^SA tumor-bearing mice (n = 4). Data are expressed as mean ± SEM and the p values were calculated using unpaired two-sided Student’s t test. c Expression heatmap of significantly affected genes (FDR < 5%) for different fold change thresholds. d List of canonical pathways downregulated that were determined by Ingenuity Pathway Analysis (IPA) as significantly enriched pathways affected by Ifnar1^SA. N = number of genes; p value = enrichment; FDR = false discovery rate of significant genes (FDR < 10%) for different fold change thresholds; Z = activation z-scores calculated by IPA represent predicted canonical pathway decreasing (Z threshold of 2 is used to call the state). A panel of top genes affected by Ifnar1^SA for each pathway. Fisher exact test was used to calculate FDR.