Fig. 10: ASXL1-MT enforces age-associated gene expression patterns. | Nature Communications

Fig. 10: ASXL1-MT enforces age-associated gene expression patterns.

From: Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway

Fig. 10: ASXL1-MT enforces age-associated gene expression patterns.The alternative text for this image may have been generated using AI.

a Volcano plot displaying differentially expressed genes in LT-HSCs between aged Vav-Cre ASXL1-MT KI mice and aged-matched control mice (n = 3). b The heatmap of the top 500 most variable genes (n = 3). c GSEA using genesets up-regulated by PTEN deficiency (PTEN_DN.V1_UP) and negatively regulated by mTOR signaling (MTOR_UP.N4.V1_DN). d Up-regulated or down-regulated genesets (MSigDB C2_CGP: chemical and genetic perturbations) in LT-HSCs from aged Vav-Cre ASXL1-MT KI mice. Up-regulated genesets involved in Akt/mTOR signaling, platelet signature, and senescence signature are shown with orange circles. Down-regulated genesets involved in myeloid differenciation, erythroid differentiation, and HSC signature are shown with blue circles. e Venn diagrams showing up-regulated or down-regulated genes in LT-HSCs of (1) aged Vav-Cre ASXL1-MT KI mice compared to aged control mice and (2) aged wild-type mice compared to young wild-type mice (GSE48893). Statistical significances are assessed by two-sided Fisher’s exact test. f GSEA using a geneset up-regulated in LT-HSCs with age (GSE48893).

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