Fig. 4: ASXL1-MT activates Akt/mTOR pathway in both young and aged mice. | Nature Communications

Fig. 4: ASXL1-MT activates Akt/mTOR pathway in both young and aged mice.

From: Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway

Fig. 4: ASXL1-MT activates Akt/mTOR pathway in both young and aged mice.The alternative text for this image may have been generated using AI.

a Gene set enrichment analysis of LSK cells from young Vav-Cre ASXL1-MT KI mice and littermate control mice using genesets up-regulated by PTEN deficiency (PTEN_DN.V1_UP) and negatively regulated by mTOR signaling (MTOR_UP.N4.V1_DN)(n = 3). b, c Levels of phosphorylated Akt (S473) (b) and phorphorylated S6 (c) in LK cells, LSK cells, MPPs, ST-HSCs and LT-HSCs from young Vav-Cre ASXL1-MT KI mice (n = 7). Representative FACS plot (left panel) and summarized data (right panel) are shown. d, e Levels of phosphorylated Akt (S473) (d) and phosphorylated S6 (e) in LT-HSCs of young and aged Vav-Cre ASXL1-MT KI mice (n = 4). Statistical significances are assessed by two-tailed Mann–Whitney’s u-test (b, c) or one-way ANOVA with Tukey–Kramer’s post-hoc test (d, e). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.

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