Fig. 3: Orion is extracellularly present on MB γ axons. | Nature Communications

Fig. 3: Orion is extracellularly present on MB γ axons.

From: Axonal chemokine-like Orion induces astrocyte infiltration and engulfment during mushroom body neuronal remodeling

Fig. 3: Orion is extracellularly present on MB γ axons.The alternative text for this image may have been generated using AI.

a–k Six-hour APF γ axons are visualized by the expression of 201Y-GAL4-driven UAS-mCD8-GFP (green). a, b, j, k γ axons expressing the wild-type Orion-B-Myc protein (red) (n = 10 MBs). c γ axons expressing the Orion-B-Myc protein lacking the signal peptide (∆SP) (n = 9 MBs). a–c are confocal Z-projections and j is a unique confocal plane. d, g Higher magnification images of the region indicated by a rectangle shows a representative unique confocal plane. Note the presence of Myc-labeled Orion-B outside the γ axon bundle (arrows). e, h Higher magnification images of the region indicated by a rectangle in b showing a representative unique confocal plane. Note the presence of Myc-labeled Orion-B inside the hole-like structures present in the γ axon bundle (arrowheads). f, i Higher magnification images of the vertical and medial γ lobes, respectively (rectangles in c). Orion-B-∆SP-Myc is observed neither outside the γ axons (f) nor in the hole-like structures (arrowheads in i). j, k Presence of Myc-labeled Orion-B extracellular proteins not associated with GFP-labeled axon membranes can be observed outside the γ axon bundle (arrows). k Three-dimensional surface-rendering (3D) of the confocal image. j Reveals close apposition of GFP-labeled axons and Myc-labeled Orion and reveals Orion is present as small extracellular globules. Scale bars represent 40 µm in a–c, 20 µm in d–i and 5 µm in j, k. Full genotypes are listed in the Supplementary list of fly strains.

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