Table 1 Unpruned axon and axon debris quantitation.

From: Axonal chemokine-like Orion induces astrocyte infiltration and engulfment during mushroom body neuronal remodeling

(a) Presence of unpruned axons in ≥1-week-old adults

 

MB

None

Weak

Strong

WT

25

25

0

0

Hr39

22

0

0

22

orionΔC

22

0

22

0

orion1

20

0

20

0

orion RNAi

34

0

34

0

drprΔ5

22

20

2

0

(b) Presence of axon debris in ≥1-week-old adults

 

MB

None

Mild

Intermediary

Strong

WT

25

25

0

0

0

Hr39

22

22

0

0

0

orionΔC

22

0

0

0

22

orion1

20

0

0

0

20

orion RNAi

34

34

0

0

0

drprΔ5

22

16

2

2

2

(c) Presence of axon debris in ≤2-h-old adults

 

MB

Scattered dots

Mild

Intermediary

Strong

WT

10

10

0

0

0

orionΔC

12

0

0

0

12

drprΔ5

73

40

11

4

18

  1. Genotypes are indicated on the left. “MB” indicates the number of mushroom bodies observed for each genotype. Unpruned axons were ranked into three categories: “None” indicates the absence of unpruned γ axons and “Weak” and “Strong” refer to different levels of the mutant pruning phenotype. Axon debris were ranked into five categories: “None” indicates the absence of debris and “Scattered dots” means that some individual debris can be observed. “Mild,” “Intermediate,” and “Strong” refer to different levels of debris (see Supplementary Fig. 2 and “Methods”). Full genotypes are listed in the Supplementary list of fly strains.